Research

My research focuses on the taxonomy and biogeography of testate amoebae, specifically targeting pseudocryptic and semi-cryptic species that form species complexes. To detect and define these species, I perform detailed morphometric analyses of morphological variability at both intra- and inter-population levels. In addition to traditional taxonomic methods, I utilize scanning electron microscopy and molecular barcoding to enhance species identification. A significant aspect of my work is the exploration of poorly studied geographical regions, including the Dinaric Alps in Southeast Europe, as well as various mountains and islands in East and Southeast Asia. These regions present unique ecological contexts that contribute to the diversity of testate amoebae. Through this comprehensive approach, I frequently discover rare, endemic, and new species, providing valuable insights into biodiversity and biogeographical patterns in these underexplored areas.

My main motivation is the incredible diversity of life. The biodiversity we see around us today is not static. It is constantly changing, evolving. Species diversify or go extinct. Various processes of natural selection are responsible for these changes, some of which are accelerated today, as we humans create changes in the environment that set up novel selection regimes. My research is focused on the evolutionary change and diversification in testate amoebae, a fascinating group of unicellular organisms that play a crucial role in ecosystems. These amoebae are known for their protective shells, which vary in shape and size, making them an interesting subject for studying morphological diversity. Their diverse habitats range from freshwater to soil and mosses, highlighting their adaptability and ecological significance. My research is distinctive in that it primarily focuses on testate amoebae inhabiting green mosses, which represent a poorly studied ecological group of these protists.

My work primarily centers on the morphometric analysis of testate amoebae, a fascinating group of protists that play significant roles in various ecosystems. By examining morphological variability both within and between populations, I strive to uncover critical insights into species differentiation and evolutionary relationships. I conduct detailed measurements of various morphological traits of testate amoebae, which allows me to assess variability within populations (intrapopulational variability) and among different populations (interpopulational variability). This approach is particularly enlightening, as intrapopulational variability reveals how different genotypes exhibit phenotypic plasticity under varying ecological conditions. In contrast, interpopulational variability helps me compare morphological characteristics across populations, shedding light on distinct evolutionary pathways and colonization histories. Such differences often reflect adaptations to specific ecological niches, providing a deeper understanding of how these organisms evolve and thrive in diverse environments.

One significant aspect of my work is identifying pseudocryptic and semi-cryptic species—terms used to describe those species that display subtle morphological differences that can complicate accurate identification. These species are vital for understanding biodiversity and ecological dynamics, as they often play significant roles in their ecosystems despite their elusive nature. Through meticulous morphometric analyses, I aim to refine taxonomic definitions and potentially uncover new species within the diverse realm of testate amoebae. By enhancing our understanding of these subtle variations, I contribute to a more nuanced view of biodiversity, highlighting the importance of each species in ecological interactions.

In addition to morphometric approaches, I employ molecular barcoding methods to enhance the accuracy of species identification. This genetic analysis allows for precise classification based on DNA sequences, providing a reliable framework for understanding evolutionary relationships among testate amoebae. By integrating genetic information with morphological data, I create a more robust and comprehensive understanding of species diversity. Another powerful tool in my research is scanning electron microscopy (SEM). This technique enables me to capture ultramicroscopic images of the amoebae’s shells, revealing intricate morphological features that standard microscopes may overlook. The detailed images obtained through SEM not only enrich our understanding of the structural diversity of testate amoebae but also contribute to more accurate taxonomic classifications.

By combining morphometric analyses, molecular barcoding, and advanced imaging techniques, I aim to unravel the complexity of testate amoebae. My research seeks to illuminate their ecological roles and contributions within their respective ecosystems. This multi-faceted approach not only enhances our understanding of these intriguing organisms but also underscores the importance of accurate taxonomy in biodiversity conservation efforts.

My research delves into the biogeography of testate amoebae, focusing on understanding their geographical distribution patterns, particularly for rare and endemic species. Current literature outlines two primary models for the distribution of these fascinating organisms: the ubiquity model, which posits that many testate amoebae are cosmopolitan, and the moderate endemicity model, which recognizes that some species are geographically restricted. These distribution patterns reveal much about the ecological dynamics at play and provide insights into the evolutionary processes that shape biodiversity.

Rare and endemic species, often referred to as the “cherries on biodiversity’s cake,” are of particular interest. These species, confined to small geographical areas, are highly vulnerable and should be prioritized for conservation efforts. Their limited range raises important questions regarding extinction and evolution. For instance, ancient endemics may offer clues about past ecological conditions, while novel endemics can shed light on recent evolutionary changes.

My research journey has taken me across diverse ecosystems, from equatorial tropical forests to near-boreal regions. This broad geographic scope allows me to explore the ecological factors that influence the presence and distribution of testate amoebae. By combining field studies with data analysis, I aim to map their distribution patterns, providing a comprehensive overview of their biogeography. This nuanced approach emphasizes the ecological significance of testate amoebae within their environments, as they serve as indicators of ecosystem health and biodiversity.

Understanding these distribution patterns is critical for informing conservation strategies and policy decisions. By identifying areas with high concentrations of rare or endemic species, we can advocate for targeted conservation actions that protect these vital components of our natural heritage. My work underscores the importance of raising awareness about testate amoebae and their ecological roles, emphasizing the need for proactive measures to preserve the ecosystems they inhabit.